There is an increasing demand for large quantities of human and/or other mammalian proteins to support drug discovery and development efforts. These proteins are not only valued in the drug screening/discovery process but are now becoming increasingly important as direct therapeutic agents.
As most therapeutically relevant proteins are only expressed in small quantities in their natural environment, the need for expression of these proteins at high levels has become increasingly important. This course will describe the strategies and techniques one can utilize to maximize protein expression in heterologous systems. The latter systems include E.coli, baculovirus, mammalian and alternative hosts. Specific examples and case studies will be given to demonstrate the capabilities and limitations of each platform
Participants will learn how to choose an appropriate host system, the best choice of vector and cell lines, and the fundamental technology needed to implement the platforms in their own laboratories. Those already familiar with these platforms will be able to benefit as new strategies will be presented to familiarize them with improved expression activities and troubleshooting techniques.
Who Should Attend
This protein expression training course will be valuable for those in the pharmaceutical and biotechnology industries as well as academics who are responsible for, or involved in, utilizing heterologous protein expression systems for recombinant protein production. Research & Development personnel will benefit as the course details all techniques and methodology to express and purify recombinant proteins of therapeutic interest.
Upon completion of this course you will:
- Understand the fundamental requirements for expression of heterologous proteins at the molecular level
- Be able to identify the best choice of vectors, expression hosts and expression strategies suited to your particular needs
- Develop an understanding of the capabilities and challenges of all available protein expression platforms
- Be able to implement hi-throughput technologies in your laboratory
- Have an understanding of techniques and strategies to utilize in order to improve protein expression levels and subsequent purification
The course will use small workshops and discussions to enable delegates to exchange ideas and experiences while also learning from each other’s experiences.